SYTO11 staining vs FISH staining: a comparison of two methods to stain Wolbachia pipientis in cell cultures
نویسندگان
چکیده
منابع مشابه
Two Step Cold Staining Methods
In developing countries pulmonary tuberculosis is usually diagnosed by detecting acid-fast bacilli (AFB) in sputum using a Ziehl-Neelsen (Z-N) staining method. However, in the field the traditional method of staining is difficult to carry out. This study evaluates the efficiency of two cold staining methods, namely Gabbet’s and a modified two reagent cold staining method compared with the Z-N t...
متن کاملP-97: A Novel Staining Method of MouseOvarian Tissue for Comparison of Two Different Methods of Vitrification
Background: Different cryoprotectants are used for Cryopreservation of ovarian tissue in patients who are at risk of infertility. Ethylene glycol (EG), dimethyl sulfoxide (DMSO) and propanediol (PROH) were chosen as the basic permeable cryoprotectants due to their less glass-formation characteristic compared to the others. In the present study, the effects of two different vitrification methods...
متن کاملA Contribution to Staining Methods
The following method for staining connective tissue is simple and is believed to be better than any yet proposed for that purpose. It is not absolutely differential because, besides connective-tissue fibrill~e and reticulum, it also stains certain hyaline substances, but these latter usually are so different morphologically that confusion cannot arise. The method is also useful for the study of...
متن کاملWolbachia pipientis growth kinetics and susceptibilities to 13 antibiotics determined by immunofluorescence staining and real-time PCR.
Wolbachia spp. are strict intracellular bacteria that infect a wide range of arthropods and filarial nematodes. Filarial nematodes are important causes of human diseases. There is increasing evidence that Wolbachia spp. influence important functions in the biology of the hosts, specifically, infertility. Preliminary experiments with humans and animals have suggested that antibiotics with activi...
متن کاملRapid Staining of Malarial Parasites by a Water Soluble Stain
In recent years it has become increasingly difficult to purchase ingredients for the preparation of various modifications of Romanowsky stains, such as Giemsa, Leishman and Wright's, at a reasonable cost; if indeed they can be obtained at all. During the past year, work has been in progress in the laboratories of the Malaria Institute of India with the object of producing a stain in which readi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2011
ISSN: 0266-8254
DOI: 10.1111/j.1472-765x.2010.02986.x